Cargando…
In vivo expression and purification of aptamer-tagged small RNA regulators
Small non-coding RNAs (sRNAs) are an emerging class of post-transcriptional regulators of bacterial gene expression. To study sRNAs and their potential protein interaction partners, it is desirable to purify sRNAs from cells in their native form. Here, we used RNA-based affinity chromatography to pu...
Autores principales: | Said, Nelly, Rieder, Renate, Hurwitz, Robert, Deckert, Jochen, Urlaub, Henning, Vogel, Jörg |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777422/ https://www.ncbi.nlm.nih.gov/pubmed/19726584 http://dx.doi.org/10.1093/nar/gkp719 |
Ejemplares similares
-
Selection of aptamers for a protein target in cell lysate and their application to protein purification
por: Javaherian, Sahar, et al.
Publicado: (2009) -
An optimized streptavidin-binding RNA aptamer for purification of ribonucleoprotein complexes identifies novel ARE-binding proteins
por: Leppek, Kathrin, et al.
Publicado: (2014) -
SnAvi – a new tandem tag for high-affinity protein-complex purification
por: Schäffer, Ursula, et al.
Publicado: (2010) -
The ARiBo tag: a reliable tool for affinity purification of RNAs under native conditions
por: Di Tomasso, Geneviève, et al.
Publicado: (2011) -
In silico selection of RNA aptamers
por: Chushak, Yaroslav, et al.
Publicado: (2009)