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Pitfalls in the normalization of real-time polymerase chain reaction data

Real-time polymerase chain reaction (PCR) is commonly used for a sensitive and specific quantification of messenger RNA (mRNA). The levels of mRNA are frequently compared between two or more experimental groups. However, such comparisons require normalization procedures, and reference genes are freq...

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Detalles Bibliográficos
Autores principales: Hendriks-Balk, M. C., Michel, M. C., Alewijnse, A. E.
Formato: Texto
Lenguaje:English
Publicado: Steinkopff-Verlag 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779446/
https://www.ncbi.nlm.nih.gov/pubmed/17370033
http://dx.doi.org/10.1007/s00395-007-0649-0