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Restriction Site-dependent PCR: An Efficient Technique for Fast Cloning of New Genes of Microorganisms
New bioactive proteins need to be screened from various microorganisms for the increasing need for industrial and pharmaceutical peptide, proteins, or enzymes. A novel polymerase chain reaction (PCR) method, restriction site-dependent PCR (RSD-PCR), was designed for rapid new genes cloning from geno...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779911/ https://www.ncbi.nlm.nih.gov/pubmed/18086803 http://dx.doi.org/10.1093/dnares/dsm023 |