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Analysis of nucleoside-binding proteins by ligand-specific elution from dye resin: application to Mycobacterium tuberculosis aldehyde dehydrogenases

We show that Cibacron Blue F3GA dye resin chromatography can be used to identify ligands that specifically interact with proteins from Mycobacterium tuberculosis, and that the identification of these ligands can facilitate structure determination by enhancing the quality of crystals. Four native Mtb...

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Detalles Bibliográficos
Autores principales: Kim, Chang-Yub, Webster, Cecelia, Roberts, Justin K. M., Moon, Jin Ho, Alipio Lyon, Emily Z., Kim, Heungbok, Yu, Minmin, Hung, Li-Wei, Terwilliger, Thomas C.
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2780684/
https://www.ncbi.nlm.nih.gov/pubmed/19911309
http://dx.doi.org/10.1007/s10969-009-9073-z