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Automated Design of Synthetic Ribosome Binding Sites to Precisely Control Protein Expression

Microbial engineering often requires fine control over protein expression; for example, to connect genetic circuits 1-7 or control flux through a metabolic pathway 8-13. We have developed a predictive design method for synthetic ribosome binding sites that enables the rational control of a protein&#...

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Detalles Bibliográficos
Autores principales: Salis, Howard M., Mirsky, Ethan A., Voigt, Christopher A.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782888/
https://www.ncbi.nlm.nih.gov/pubmed/19801975
http://dx.doi.org/10.1038/nbt.1568