TU-tagging: cell type specific RNA isolation from intact complex tissues

We show that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil (4TU) delivery can be used to label and purify cell type specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell ty...

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Detalles Bibliográficos
Autores principales: Miller, Michael R., Robinson, Kristin J., Cleary, Michael D., Doe, Chris Q.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2783170/
https://www.ncbi.nlm.nih.gov/pubmed/19430475
http://dx.doi.org/10.1038/nmeth.1329

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2783170/
https://www.ncbi.nlm.nih.gov/pubmed/19430475
http://dx.doi.org/10.1038/nmeth.1329

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