TU-tagging: cell type specific RNA isolation from intact complex tissues

We show that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil (4TU) delivery can be used to label and purify cell type specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell ty...

Descripción completa

Detalles Bibliográficos
Autores principales: Miller, Michael R., Robinson, Kristin J., Cleary, Michael D., Doe, Chris Q.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2783170/
https://www.ncbi.nlm.nih.gov/pubmed/19430475
http://dx.doi.org/10.1038/nmeth.1329
_version_ 1782174691866181632
author Miller, Michael R.
Robinson, Kristin J.
Cleary, Michael D.
Doe, Chris Q.
author_facet Miller, Michael R.
Robinson, Kristin J.
Cleary, Michael D.
Doe, Chris Q.
author_sort Miller, Michael R.
collection PubMed
description We show that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil (4TU) delivery can be used to label and purify cell type specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in many organisms, including mammals and other vertebrates.
format Text
id pubmed-2783170
institution National Center for Biotechnology Information
language English
publishDate 2009
record_format MEDLINE/PubMed
spelling pubmed-27831702009-12-01 TU-tagging: cell type specific RNA isolation from intact complex tissues Miller, Michael R. Robinson, Kristin J. Cleary, Michael D. Doe, Chris Q. Nat Methods Article We show that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil (4TU) delivery can be used to label and purify cell type specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in many organisms, including mammals and other vertebrates. 2009-05-10 2009-06 /pmc/articles/PMC2783170/ /pubmed/19430475 http://dx.doi.org/10.1038/nmeth.1329 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Miller, Michael R.
Robinson, Kristin J.
Cleary, Michael D.
Doe, Chris Q.
TU-tagging: cell type specific RNA isolation from intact complex tissues
title TU-tagging: cell type specific RNA isolation from intact complex tissues
title_full TU-tagging: cell type specific RNA isolation from intact complex tissues
title_fullStr TU-tagging: cell type specific RNA isolation from intact complex tissues
title_full_unstemmed TU-tagging: cell type specific RNA isolation from intact complex tissues
title_short TU-tagging: cell type specific RNA isolation from intact complex tissues
title_sort tu-tagging: cell type specific rna isolation from intact complex tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2783170/
https://www.ncbi.nlm.nih.gov/pubmed/19430475
http://dx.doi.org/10.1038/nmeth.1329
work_keys_str_mv AT millermichaelr tutaggingcelltypespecificrnaisolationfromintactcomplextissues
AT robinsonkristinj tutaggingcelltypespecificrnaisolationfromintactcomplextissues
AT clearymichaeld tutaggingcelltypespecificrnaisolationfromintactcomplextissues
AT doechrisq tutaggingcelltypespecificrnaisolationfromintactcomplextissues

Ejemplares similares