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APPLICATION OF POLYMERASE CHAIN REACTION (PCR) AND PCR BASED RESTRICTION FRAGMENT LENGTH POLYMORPHISM FOR DETECTION AND IDENTIFICATION OF DERMATOPHYTES FROM DERMATOLOGICAL SPECIMENS

OBJECTIVE: To develop and optimize polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) targeting 18S rDNA and internal transcribed spacer (ITS) region of fungi for rapid detection and identification of dermatophytes. MATERIALS AND METHODS: Two PCR-RFLP methods targeti...

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Detalles Bibliográficos
Autores principales: Bagyalakshmi, R, Senthilvelan, B, Therese, K L, Murugusundram, S, Madhavan, H N
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2784578/
https://www.ncbi.nlm.nih.gov/pubmed/19967012
http://dx.doi.org/10.4103/0019-5154.39735