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A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
This is a method for zebrafish sperm cryopreservation that is an adaptation of the Harvey method (Harvey et al., 1982). We have introduced two changes to the original protocol that both streamline the procedure and increase sample uniformity. First, we normalize all sperm volumes using freezing medi...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
MyJove Corporation
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2785217/ https://www.ncbi.nlm.nih.gov/pubmed/19581874 http://dx.doi.org/10.3791/1395 |