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A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

This is a method for zebrafish sperm cryopreservation that is an adaptation of the Harvey method (Harvey et al., 1982). We have introduced two changes to the original protocol that both streamline the procedure and increase sample uniformity. First, we normalize all sperm volumes using freezing medi...

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Detalles Bibliográficos
Autores principales:	Draper, Bruce W., Moens, Cecilia B.
Formato:	Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2009
Materias:	Developmental Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2785217/ https://www.ncbi.nlm.nih.gov/pubmed/19581874 http://dx.doi.org/10.3791/1395

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