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Fluorescent Duplex Allele-Specific PCR and Amplicon Melting for Rapid Homogeneous mtDNA Haplogroup H Screening and Sensitive Mixture Detection

BACKGROUND: For large scale studies aiming at a better understanding of mitochondrial DNA (mtDNA), sequence variation in particular mt haplogroups (hgs) and population structure, reliable low-cost high-throughput genotyping assays are needed. Furthermore, methods facilitating sensitive mixture detec...

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Detalles Bibliográficos
Autores principales: Niederstätter, Harald, Parson, Walther
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793010/
https://www.ncbi.nlm.nih.gov/pubmed/20020064
http://dx.doi.org/10.1371/journal.pone.0008374