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Assay and heterologous expression in Pichia pastoris of plant cell wall type-II membrane anchored glycosyltransferases

Two Arabidopsis xylosyltransferases, designated RGXT1 and RGXT2, were recently expressed in Baculovirus transfected insect cells and by use of the free sugar assay shown to catalyse transfer of D-xylose from UDP-α-D-xylose to L-fucose and derivatives hereof. We have now examined expression of RGXT1...

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Detalles Bibliográficos
Autores principales: Petersen, Bent Larsen, Egelund, Jack, Damager, Iben, Faber, Kirsten, Krüger Jensen, Jacob, Yang, Zhang, Bennett, Eric Paul, Scheller, Henrik Vibe, Ulvskov, Peter
Formato: Texto
Lenguaje:English
Publicado: Springer US 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793385/
https://www.ncbi.nlm.nih.gov/pubmed/19455420
http://dx.doi.org/10.1007/s10719-009-9242-0