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Assay and heterologous expression in Pichia pastoris of plant cell wall type-II membrane anchored glycosyltransferases
Two Arabidopsis xylosyltransferases, designated RGXT1 and RGXT2, were recently expressed in Baculovirus transfected insect cells and by use of the free sugar assay shown to catalyse transfer of D-xylose from UDP-α-D-xylose to L-fucose and derivatives hereof. We have now examined expression of RGXT1...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer US
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793385/ https://www.ncbi.nlm.nih.gov/pubmed/19455420 http://dx.doi.org/10.1007/s10719-009-9242-0 |