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The catalytic residues of Tn3 resolvase

To characterize the residues that participate in the catalysis of DNA cleavage and rejoining by the site-specific recombinase Tn3 resolvase, we mutated conserved polar or charged residues in the catalytic domain of an activated resolvase variant. We analysed the effects of mutations at 14 residues o...

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Detalles Bibliográficos
Autores principales: Olorunniji, Femi J., Stark, W. Marshall
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2794168/
https://www.ncbi.nlm.nih.gov/pubmed/19789272
http://dx.doi.org/10.1093/nar/gkp797