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The catalytic residues of Tn3 resolvase
To characterize the residues that participate in the catalysis of DNA cleavage and rejoining by the site-specific recombinase Tn3 resolvase, we mutated conserved polar or charged residues in the catalytic domain of an activated resolvase variant. We analysed the effects of mutations at 14 residues o...
Autores principales: | Olorunniji, Femi J., Stark, W. Marshall |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2794168/ https://www.ncbi.nlm.nih.gov/pubmed/19789272 http://dx.doi.org/10.1093/nar/gkp797 |
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