Introducing a true internal standard for the Comet assay to minimize intra- and inter-experiment variability in measures of DNA damage and repair

The Comet assay (CA) is a sensitive/simple measure of genotoxicity. However, many features of CA contribute variability. To minimize these, we have introduced internal standard materials consisting of ‘reference’ cells which have their DNA substituted with BrdU. Using a fluorescent anti-BrdU antibod...

Descripción completa

Detalles Bibliográficos
Autores principales: Zainol, Murizal, Stoute, Julia, Almeida, Gabriela M., Rapp, Alexander, Bowman, Karen J., Jones, George D. D.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2794182/
https://www.ncbi.nlm.nih.gov/pubmed/19828597
http://dx.doi.org/10.1093/nar/gkp826

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2794182/
https://www.ncbi.nlm.nih.gov/pubmed/19828597
http://dx.doi.org/10.1093/nar/gkp826

Ejemplares similares