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Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.Csp231I from Citrobacter sp. RFL231

Restriction–modification controller proteins play an essential role in regulating the temporal expression of restriction–modification genes. The controller protein C.Csp231I represents a new class of controller proteins. The gene was sublconed to allow overexpression in Escherichia coli. The protein...

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Detalles Bibliográficos
Autores principales: Streeter, S. D., McGeehan, J. E., Kneale, G. G.
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2795595/
https://www.ncbi.nlm.nih.gov/pubmed/19724127
http://dx.doi.org/10.1107/S1744309109028681