Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.Csp231I from Citrobacter sp. RFL231

Restriction–modification controller proteins play an essential role in regulating the temporal expression of restriction–modification genes. The controller protein C.Csp231I represents a new class of controller proteins. The gene was sublconed to allow overexpression in Escherichia coli. The protein...

Descripción completa

Detalles Bibliográficos
Autores principales: Streeter, S. D., McGeehan, J. E., Kneale, G. G.
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2009
Materias:
Crystallization Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2795595/
https://www.ncbi.nlm.nih.gov/pubmed/19724127
http://dx.doi.org/10.1107/S1744309109028681
_version_ 1782175439159033856
author Streeter, S. D.
McGeehan, J. E.
Kneale, G. G.
author_facet Streeter, S. D.
McGeehan, J. E.
Kneale, G. G.
author_sort Streeter, S. D.
collection PubMed
description Restriction–modification controller proteins play an essential role in regulating the temporal expression of restriction–modification genes. The controller protein C.Csp231I represents a new class of controller proteins. The gene was sublconed to allow overexpression in Escherichia coli. The protein was purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.0 Å resolution and belonged to space group P2(1). An electrophoretic mobility-shift assay provided evidence of strong binding of C.Csp231I to a sequence located upstream of the csp231IC start codon.
format Text
id pubmed-2795595
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher International Union of Crystallography
record_format MEDLINE/PubMed
spelling pubmed-27955952009-12-18 Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.Csp231I from Citrobacter sp. RFL231 Streeter, S. D. McGeehan, J. E. Kneale, G. G. Acta Crystallogr Sect F Struct Biol Cryst Commun Crystallization Communications Restriction–modification controller proteins play an essential role in regulating the temporal expression of restriction–modification genes. The controller protein C.Csp231I represents a new class of controller proteins. The gene was sublconed to allow overexpression in Escherichia coli. The protein was purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.0 Å resolution and belonged to space group P2(1). An electrophoretic mobility-shift assay provided evidence of strong binding of C.Csp231I to a sequence located upstream of the csp231IC start codon. International Union of Crystallography 2009-08-22 /pmc/articles/PMC2795595/ /pubmed/19724127 http://dx.doi.org/10.1107/S1744309109028681 Text en © Streeter et al. 2009 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Crystallization Communications
Streeter, S. D.
McGeehan, J. E.
Kneale, G. G.
Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.Csp231I from Citrobacter sp. RFL231
title Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.Csp231I from Citrobacter sp. RFL231
title_full Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.Csp231I from Citrobacter sp. RFL231
title_fullStr Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.Csp231I from Citrobacter sp. RFL231
title_full_unstemmed Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.Csp231I from Citrobacter sp. RFL231
title_short Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.Csp231I from Citrobacter sp. RFL231
title_sort overexpression, purification and preliminary x-ray diffraction analysis of the controller protein c.csp231i from citrobacter sp. rfl231
topic Crystallization Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2795595/
https://www.ncbi.nlm.nih.gov/pubmed/19724127
http://dx.doi.org/10.1107/S1744309109028681
work_keys_str_mv AT streetersd overexpressionpurificationandpreliminaryxraydiffractionanalysisofthecontrollerproteinccsp231ifromcitrobactersprfl231
AT mcgeehanje overexpressionpurificationandpreliminaryxraydiffractionanalysisofthecontrollerproteinccsp231ifromcitrobactersprfl231
AT knealegg overexpressionpurificationandpreliminaryxraydiffractionanalysisofthecontrollerproteinccsp231ifromcitrobactersprfl231

Ejemplares similares