Temperature Switch PCR (TSP): Robust assay design for reliable amplification and genotyping of SNPs

BACKGROUND: Many research and diagnostic applications rely upon the assay of individual single nucleotide polymorphisms (SNPs). Thus, methods to improve the speed and efficiency for single-marker SNP genotyping are highly desirable. Here, we describe the method of temperature-switch PCR (TSP), a bip...

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Detalles Bibliográficos
Autores principales: Tabone, Tania, Mather, Diane E, Hayden, Matthew J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Methodology article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2795770/
https://www.ncbi.nlm.nih.gov/pubmed/19958555
http://dx.doi.org/10.1186/1471-2164-10-580

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2795770/
https://www.ncbi.nlm.nih.gov/pubmed/19958555
http://dx.doi.org/10.1186/1471-2164-10-580

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