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Temperature Switch PCR (TSP): Robust assay design for reliable amplification and genotyping of SNPs
BACKGROUND: Many research and diagnostic applications rely upon the assay of individual single nucleotide polymorphisms (SNPs). Thus, methods to improve the speed and efficiency for single-marker SNP genotyping are highly desirable. Here, we describe the method of temperature-switch PCR (TSP), a bip...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2009
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2795770/ https://www.ncbi.nlm.nih.gov/pubmed/19958555 http://dx.doi.org/10.1186/1471-2164-10-580 |