Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains

BACKGROUND: Homologous recombination mediated by the λ-Red genes is a common method for making chromosomal modifications in Escherichia coli. Several protocols have been developed that differ in the mechanisms by which DNA, carrying regions homologous to the chromosome, are delivered into the cell....

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Detalles Bibliográficos
Autores principales: Lee, David J, Bingle, Lewis EH, Heurlier, Karin, Pallen, Mark J, Penn, Charles W, Busby, Stephen JW, Hobman, Jon L
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Methodology article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796669/
https://www.ncbi.nlm.nih.gov/pubmed/20003185
http://dx.doi.org/10.1186/1471-2180-9-252

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796669/
https://www.ncbi.nlm.nih.gov/pubmed/20003185
http://dx.doi.org/10.1186/1471-2180-9-252

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