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Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains
BACKGROUND: Homologous recombination mediated by the λ-Red genes is a common method for making chromosomal modifications in Escherichia coli. Several protocols have been developed that differ in the mechanisms by which DNA, carrying regions homologous to the chromosome, are delivered into the cell....
Autores principales: | Lee, David J, Bingle, Lewis EH, Heurlier, Karin, Pallen, Mark J, Penn, Charles W, Busby, Stephen JW, Hobman, Jon L |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796669/ https://www.ncbi.nlm.nih.gov/pubmed/20003185 http://dx.doi.org/10.1186/1471-2180-9-252 |
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