Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

The preparation of single-cell suspensions from tissues is an important prerequisite for many experiments in cellular research. The process of dissociating whole organs requires specific parameters in order to obtain a high number of viable cells in a reproducible manner. The gentleMACS Dissociator...

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Detalles Bibliográficos
Autores principales: Jungblut, Melanie, Oeltze, Karen, Zehnter, Irene, Hasselmann, Doris, Bosio, Andreas
Formato: Texto
Lenguaje:English
Publicado: MyJove Corporation 2009
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2798855/
https://www.ncbi.nlm.nih.gov/pubmed/19574953
http://dx.doi.org/10.3791/1266

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2798855/
https://www.ncbi.nlm.nih.gov/pubmed/19574953
http://dx.doi.org/10.3791/1266

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