Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

The preparation of single-cell suspensions from tissues is an important prerequisite for many experiments in cellular research. The process of dissociating whole organs requires specific parameters in order to obtain a high number of viable cells in a reproducible manner. The gentleMACS Dissociator...

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Detalles Bibliográficos
Autores principales: Jungblut, Melanie, Oeltze, Karen, Zehnter, Irene, Hasselmann, Doris, Bosio, Andreas
Formato: Texto
Lenguaje:English
Publicado: MyJove Corporation 2009
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2798855/
https://www.ncbi.nlm.nih.gov/pubmed/19574953
http://dx.doi.org/10.3791/1266
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author Jungblut, Melanie
Oeltze, Karen
Zehnter, Irene
Hasselmann, Doris
Bosio, Andreas
author_facet Jungblut, Melanie
Oeltze, Karen
Zehnter, Irene
Hasselmann, Doris
Bosio, Andreas
author_sort Jungblut, Melanie
collection PubMed
description The preparation of single-cell suspensions from tissues is an important prerequisite for many experiments in cellular research. The process of dissociating whole organs requires specific parameters in order to obtain a high number of viable cells in a reproducible manner. The gentleMACS Dissociator optimizes this task with a simple, practical protocol. The instrument contains pre-programmed settings that are optimized for the efficient but gentle dissociation of a variety of tissue types, including mouse lungs. In this publication the use of the gentleMACS Dissociator on lung tissue derived from mice is demonstrated.
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spelling pubmed-27988552011-07-02 Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator Jungblut, Melanie Oeltze, Karen Zehnter, Irene Hasselmann, Doris Bosio, Andreas J Vis Exp Cell Biology The preparation of single-cell suspensions from tissues is an important prerequisite for many experiments in cellular research. The process of dissociating whole organs requires specific parameters in order to obtain a high number of viable cells in a reproducible manner. The gentleMACS Dissociator optimizes this task with a simple, practical protocol. The instrument contains pre-programmed settings that are optimized for the efficient but gentle dissociation of a variety of tissue types, including mouse lungs. In this publication the use of the gentleMACS Dissociator on lung tissue derived from mice is demonstrated. MyJove Corporation 2009-07-02 /pmc/articles/PMC2798855/ /pubmed/19574953 http://dx.doi.org/10.3791/1266 Text en Copyright © 2009, Journal of Visualized Experiments http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cell Biology
Jungblut, Melanie
Oeltze, Karen
Zehnter, Irene
Hasselmann, Doris
Bosio, Andreas
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
title Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
title_full Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
title_fullStr Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
title_full_unstemmed Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
title_short Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
title_sort standardized preparation of single-cell suspensions from mouse lung tissue using the gentlemacs dissociator
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2798855/
https://www.ncbi.nlm.nih.gov/pubmed/19574953
http://dx.doi.org/10.3791/1266
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