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Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
The preparation of single-cell suspensions from tissues is an important prerequisite for many experiments in cellular research. The process of dissociating whole organs requires specific parameters in order to obtain a high number of viable cells in a reproducible manner. The gentleMACS Dissociator...
Autores principales: | Jungblut, Melanie, Oeltze, Karen, Zehnter, Irene, Hasselmann, Doris, Bosio, Andreas |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2798855/ https://www.ncbi.nlm.nih.gov/pubmed/19574953 http://dx.doi.org/10.3791/1266 |
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