Understanding how the crowded interior of cells stabilizes DNA/DNA and DNA/RNA hybrids–in silico predictions and in vitro evidence

Amplification of DNA in vivo occurs in intracellular environments characterized by macromolecular crowding (MMC). In vitro Polymerase-chain-reaction (PCR), however, is non-crowded, requires thermal cycling for melting of DNA strands, primer-template hybridization and enzymatic primer-extension. The...

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Detalles Bibliográficos
Autores principales: Harve, Karthik S., Lareu, Ricky, Rajagopalan, Raj, Raghunath, Michael
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2800234/
https://www.ncbi.nlm.nih.gov/pubmed/19854935
http://dx.doi.org/10.1093/nar/gkp884

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2800234/
https://www.ncbi.nlm.nih.gov/pubmed/19854935
http://dx.doi.org/10.1093/nar/gkp884

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