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5-Lipoxygenase DNA Methylation and mRNA Content in the Brain and Heart of Young and Old Mice

The expression of 5-lipoxygenase (5-LOX) is affected by aging and regulated by epigenetic mechanisms including DNA methylation. We used methylation-sensitive restriction endonucleases (AciI, BstUI, HpaII, and HinP1I) to assess 5-LOX DNA methylation in brain and heart tissue samples from young (2 mon...

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Autores principales: Dzitoyeva, Svetlana, Imbesi, Marta, Ng, Louisa W., Manev, Hari
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2801004/
https://www.ncbi.nlm.nih.gov/pubmed/20052386
http://dx.doi.org/10.1155/2009/209596
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author Dzitoyeva, Svetlana
Imbesi, Marta
Ng, Louisa W.
Manev, Hari
author_facet Dzitoyeva, Svetlana
Imbesi, Marta
Ng, Louisa W.
Manev, Hari
author_sort Dzitoyeva, Svetlana
collection PubMed
description The expression of 5-lipoxygenase (5-LOX) is affected by aging and regulated by epigenetic mechanisms including DNA methylation. We used methylation-sensitive restriction endonucleases (AciI, BstUI, HpaII, and HinP1I) to assess 5-LOX DNA methylation in brain and heart tissue samples from young (2 months) and old (22 months) mice. We also measured mRNA content for 5-LOX and the DNA methyltransferases DNMT1 and DNMT3a. In young mice, the 5-LOX mRNA content was significantly greater in the heart compared to the brain; 5-LOX DNA methylation was lower, except in the AciI assay in which it was higher in the heart. Aging decreased 5-LOX mRNA content in the heart and increased it in the brain. Aging also increased 5-LOX DNA methylation and this effect was site- (i.e., enzyme) and tissue-specific. Generally, DNMT1 and DNMT3a mRNA content was lower in the brain regions compared to the heart; the only effect of aging was observed in the mRNA content of DNMT3a, which was decreased in the heart of old mice. These results indicate a complex tissue-specific and aging-dependent interplay between the DNA methylation system and 5-LOX mRNA content. Interpretation of this data must take into account that the tissue samples contained a mixture of various cell types.
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spelling pubmed-28010042010-01-05 5-Lipoxygenase DNA Methylation and mRNA Content in the Brain and Heart of Young and Old Mice Dzitoyeva, Svetlana Imbesi, Marta Ng, Louisa W. Manev, Hari Neural Plast Research Article The expression of 5-lipoxygenase (5-LOX) is affected by aging and regulated by epigenetic mechanisms including DNA methylation. We used methylation-sensitive restriction endonucleases (AciI, BstUI, HpaII, and HinP1I) to assess 5-LOX DNA methylation in brain and heart tissue samples from young (2 months) and old (22 months) mice. We also measured mRNA content for 5-LOX and the DNA methyltransferases DNMT1 and DNMT3a. In young mice, the 5-LOX mRNA content was significantly greater in the heart compared to the brain; 5-LOX DNA methylation was lower, except in the AciI assay in which it was higher in the heart. Aging decreased 5-LOX mRNA content in the heart and increased it in the brain. Aging also increased 5-LOX DNA methylation and this effect was site- (i.e., enzyme) and tissue-specific. Generally, DNMT1 and DNMT3a mRNA content was lower in the brain regions compared to the heart; the only effect of aging was observed in the mRNA content of DNMT3a, which was decreased in the heart of old mice. These results indicate a complex tissue-specific and aging-dependent interplay between the DNA methylation system and 5-LOX mRNA content. Interpretation of this data must take into account that the tissue samples contained a mixture of various cell types. Hindawi Publishing Corporation 2009 2009-12-13 /pmc/articles/PMC2801004/ /pubmed/20052386 http://dx.doi.org/10.1155/2009/209596 Text en Copyright © 2009 Svetlana Dzitoyeva et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dzitoyeva, Svetlana
Imbesi, Marta
Ng, Louisa W.
Manev, Hari
5-Lipoxygenase DNA Methylation and mRNA Content in the Brain and Heart of Young and Old Mice
title 5-Lipoxygenase DNA Methylation and mRNA Content in the Brain and Heart of Young and Old Mice
title_full 5-Lipoxygenase DNA Methylation and mRNA Content in the Brain and Heart of Young and Old Mice
title_fullStr 5-Lipoxygenase DNA Methylation and mRNA Content in the Brain and Heart of Young and Old Mice
title_full_unstemmed 5-Lipoxygenase DNA Methylation and mRNA Content in the Brain and Heart of Young and Old Mice
title_short 5-Lipoxygenase DNA Methylation and mRNA Content in the Brain and Heart of Young and Old Mice
title_sort 5-lipoxygenase dna methylation and mrna content in the brain and heart of young and old mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2801004/
https://www.ncbi.nlm.nih.gov/pubmed/20052386
http://dx.doi.org/10.1155/2009/209596
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