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Antioxidant Activity of a Red Lentil Extract and Its Fractions
Phenolic compounds were extracted from red lentil seeds using 80% (v/v) aqueous acetone. The crude extract was applied to a Sephadex LH-20 column. Fraction 1, consisting of sugars and low-molecular-weight phenolics, was eluted from the column by ethanol. Fraction 2, consisting of tannins, was obtain...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2802008/ https://www.ncbi.nlm.nih.gov/pubmed/20054484 http://dx.doi.org/10.3390/ijms10125513 |
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author | Amarowicz, Ryszard Estrella, Isabell Hernández, Teresa Dueñas, Montserrat Troszyńska, Agnieszka Agnieszka, Kosińska Pegg, Ronald B. |
author_facet | Amarowicz, Ryszard Estrella, Isabell Hernández, Teresa Dueñas, Montserrat Troszyńska, Agnieszka Agnieszka, Kosińska Pegg, Ronald B. |
author_sort | Amarowicz, Ryszard |
collection | PubMed |
description | Phenolic compounds were extracted from red lentil seeds using 80% (v/v) aqueous acetone. The crude extract was applied to a Sephadex LH-20 column. Fraction 1, consisting of sugars and low-molecular-weight phenolics, was eluted from the column by ethanol. Fraction 2, consisting of tannins, was obtained using acetone-water (1:1; v/v) as the mobile phase. Phenolic compounds present in the crude extract and its fractions demonstrated antioxidant and antiradical activities as revealed from studies using a β-carotene-linoleate model system, the total antioxidant activity (TAA) method, the DPPH radical-scavenging activity assay, and a reducing power evaluation. Results of these assays showed the highest values when tannins (fraction 2) were tested. For instance, the TAA of the tannin fraction was 5.85 μmol Trolox(®) eq./mg, whereas the crude extract and fraction 1 showed 0.68 and 0.33 μmol Trolox(®) eq./mg, respectively. The content of total phenolics in fraction 2 was the highest (290 mg/g); the tannin content, determined using the vanillin method and expressed as absorbance units at 500 nm per 1 g, was 129. There were 24 compounds identified in the crude extract using an HPLC-ESI-MS method: quercetin diglycoside, catechin, digallate procyanidin, and p-hydroxybenzoic were the dominant phenolics in the extract. |
format | Text |
id | pubmed-2802008 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-28020082010-01-06 Antioxidant Activity of a Red Lentil Extract and Its Fractions Amarowicz, Ryszard Estrella, Isabell Hernández, Teresa Dueñas, Montserrat Troszyńska, Agnieszka Agnieszka, Kosińska Pegg, Ronald B. Int J Mol Sci Article Phenolic compounds were extracted from red lentil seeds using 80% (v/v) aqueous acetone. The crude extract was applied to a Sephadex LH-20 column. Fraction 1, consisting of sugars and low-molecular-weight phenolics, was eluted from the column by ethanol. Fraction 2, consisting of tannins, was obtained using acetone-water (1:1; v/v) as the mobile phase. Phenolic compounds present in the crude extract and its fractions demonstrated antioxidant and antiradical activities as revealed from studies using a β-carotene-linoleate model system, the total antioxidant activity (TAA) method, the DPPH radical-scavenging activity assay, and a reducing power evaluation. Results of these assays showed the highest values when tannins (fraction 2) were tested. For instance, the TAA of the tannin fraction was 5.85 μmol Trolox(®) eq./mg, whereas the crude extract and fraction 1 showed 0.68 and 0.33 μmol Trolox(®) eq./mg, respectively. The content of total phenolics in fraction 2 was the highest (290 mg/g); the tannin content, determined using the vanillin method and expressed as absorbance units at 500 nm per 1 g, was 129. There were 24 compounds identified in the crude extract using an HPLC-ESI-MS method: quercetin diglycoside, catechin, digallate procyanidin, and p-hydroxybenzoic were the dominant phenolics in the extract. Molecular Diversity Preservation International (MDPI) 2009-12-23 /pmc/articles/PMC2802008/ /pubmed/20054484 http://dx.doi.org/10.3390/ijms10125513 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Amarowicz, Ryszard Estrella, Isabell Hernández, Teresa Dueñas, Montserrat Troszyńska, Agnieszka Agnieszka, Kosińska Pegg, Ronald B. Antioxidant Activity of a Red Lentil Extract and Its Fractions |
title | Antioxidant Activity of a Red Lentil Extract and Its Fractions |
title_full | Antioxidant Activity of a Red Lentil Extract and Its Fractions |
title_fullStr | Antioxidant Activity of a Red Lentil Extract and Its Fractions |
title_full_unstemmed | Antioxidant Activity of a Red Lentil Extract and Its Fractions |
title_short | Antioxidant Activity of a Red Lentil Extract and Its Fractions |
title_sort | antioxidant activity of a red lentil extract and its fractions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2802008/ https://www.ncbi.nlm.nih.gov/pubmed/20054484 http://dx.doi.org/10.3390/ijms10125513 |
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