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Crude subcellular fractionation of cultured mammalian cell lines
BACKGROUND: The expression and study of recombinant proteins in mammalian culture systems can be complicated during the cell lysis procedure by contaminating proteins from cellular compartments distinct from those within which the protein of interest resides and also by solubility issues that may ar...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2009
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2802353/ https://www.ncbi.nlm.nih.gov/pubmed/20003239 http://dx.doi.org/10.1186/1756-0500-2-243 |