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A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer

We identified a sequence embedded in the U3/R region of HIV-1 RNA that is highly complementary to human tRNA(3)(Lys). The free energy of annealing to tRNA(3)(Lys) is significantly lower for this sequence and the primer-binding site than for other similar length viral sequences. The only interruption...

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Detalles Bibliográficos
Autores principales: Piekna-Przybylska, Dorota, DiChiacchio, Laura, Mathews, David H., Bambara, Robert A.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2802660/
https://www.ncbi.nlm.nih.gov/pubmed/19966801
http://dx.doi.org/10.1038/nsmb.1687
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author Piekna-Przybylska, Dorota
DiChiacchio, Laura
Mathews, David H.
Bambara, Robert A.
author_facet Piekna-Przybylska, Dorota
DiChiacchio, Laura
Mathews, David H.
Bambara, Robert A.
author_sort Piekna-Przybylska, Dorota
collection PubMed
description We identified a sequence embedded in the U3/R region of HIV-1 RNA that is highly complementary to human tRNA(3)(Lys). The free energy of annealing to tRNA(3)(Lys) is significantly lower for this sequence and the primer-binding site than for other similar length viral sequences. The only interruption in complementarity is a 29-nucleotide segment inserted where a tRNA intron would be expected. The insert contains the TATA box for viral RNA transcription. The embedded sequence includes a nine-nucleotide segment previously reported to aid minus strand transfer by binding the primer tRNA(3)(Lys). Reconstituting transfer in vitro, we show that including segments from the embedded sequence in the acceptor template, beyond the nine nucleotides, further increases transfer efficiency. We propose that a tRNA(3)(Lys) gene was incorporated during HIV-1 evolution and retained largely intact because of its roles in transcription and strand transfer.
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spelling pubmed-28026602010-07-01 A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer Piekna-Przybylska, Dorota DiChiacchio, Laura Mathews, David H. Bambara, Robert A. Nat Struct Mol Biol Article We identified a sequence embedded in the U3/R region of HIV-1 RNA that is highly complementary to human tRNA(3)(Lys). The free energy of annealing to tRNA(3)(Lys) is significantly lower for this sequence and the primer-binding site than for other similar length viral sequences. The only interruption in complementarity is a 29-nucleotide segment inserted where a tRNA intron would be expected. The insert contains the TATA box for viral RNA transcription. The embedded sequence includes a nine-nucleotide segment previously reported to aid minus strand transfer by binding the primer tRNA(3)(Lys). Reconstituting transfer in vitro, we show that including segments from the embedded sequence in the acceptor template, beyond the nine nucleotides, further increases transfer efficiency. We propose that a tRNA(3)(Lys) gene was incorporated during HIV-1 evolution and retained largely intact because of its roles in transcription and strand transfer. 2009-12-06 2010-01 /pmc/articles/PMC2802660/ /pubmed/19966801 http://dx.doi.org/10.1038/nsmb.1687 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Piekna-Przybylska, Dorota
DiChiacchio, Laura
Mathews, David H.
Bambara, Robert A.
A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer
title A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer
title_full A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer
title_fullStr A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer
title_full_unstemmed A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer
title_short A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer
title_sort sequence similar to trna(3)(lys) gene is embedded in hiv-1 u3/r and promotes minus strand transfer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2802660/
https://www.ncbi.nlm.nih.gov/pubmed/19966801
http://dx.doi.org/10.1038/nsmb.1687
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