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A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer
We identified a sequence embedded in the U3/R region of HIV-1 RNA that is highly complementary to human tRNA(3)(Lys). The free energy of annealing to tRNA(3)(Lys) is significantly lower for this sequence and the primer-binding site than for other similar length viral sequences. The only interruption...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2802660/ https://www.ncbi.nlm.nih.gov/pubmed/19966801 http://dx.doi.org/10.1038/nsmb.1687 |
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author | Piekna-Przybylska, Dorota DiChiacchio, Laura Mathews, David H. Bambara, Robert A. |
author_facet | Piekna-Przybylska, Dorota DiChiacchio, Laura Mathews, David H. Bambara, Robert A. |
author_sort | Piekna-Przybylska, Dorota |
collection | PubMed |
description | We identified a sequence embedded in the U3/R region of HIV-1 RNA that is highly complementary to human tRNA(3)(Lys). The free energy of annealing to tRNA(3)(Lys) is significantly lower for this sequence and the primer-binding site than for other similar length viral sequences. The only interruption in complementarity is a 29-nucleotide segment inserted where a tRNA intron would be expected. The insert contains the TATA box for viral RNA transcription. The embedded sequence includes a nine-nucleotide segment previously reported to aid minus strand transfer by binding the primer tRNA(3)(Lys). Reconstituting transfer in vitro, we show that including segments from the embedded sequence in the acceptor template, beyond the nine nucleotides, further increases transfer efficiency. We propose that a tRNA(3)(Lys) gene was incorporated during HIV-1 evolution and retained largely intact because of its roles in transcription and strand transfer. |
format | Text |
id | pubmed-2802660 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
record_format | MEDLINE/PubMed |
spelling | pubmed-28026602010-07-01 A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer Piekna-Przybylska, Dorota DiChiacchio, Laura Mathews, David H. Bambara, Robert A. Nat Struct Mol Biol Article We identified a sequence embedded in the U3/R region of HIV-1 RNA that is highly complementary to human tRNA(3)(Lys). The free energy of annealing to tRNA(3)(Lys) is significantly lower for this sequence and the primer-binding site than for other similar length viral sequences. The only interruption in complementarity is a 29-nucleotide segment inserted where a tRNA intron would be expected. The insert contains the TATA box for viral RNA transcription. The embedded sequence includes a nine-nucleotide segment previously reported to aid minus strand transfer by binding the primer tRNA(3)(Lys). Reconstituting transfer in vitro, we show that including segments from the embedded sequence in the acceptor template, beyond the nine nucleotides, further increases transfer efficiency. We propose that a tRNA(3)(Lys) gene was incorporated during HIV-1 evolution and retained largely intact because of its roles in transcription and strand transfer. 2009-12-06 2010-01 /pmc/articles/PMC2802660/ /pubmed/19966801 http://dx.doi.org/10.1038/nsmb.1687 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Piekna-Przybylska, Dorota DiChiacchio, Laura Mathews, David H. Bambara, Robert A. A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer |
title | A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer |
title_full | A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer |
title_fullStr | A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer |
title_full_unstemmed | A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer |
title_short | A sequence similar to tRNA(3)(Lys) gene is embedded in HIV-1 U3/R and promotes minus strand transfer |
title_sort | sequence similar to trna(3)(lys) gene is embedded in hiv-1 u3/r and promotes minus strand transfer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2802660/ https://www.ncbi.nlm.nih.gov/pubmed/19966801 http://dx.doi.org/10.1038/nsmb.1687 |
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