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Quality assessment of DNA derived from up to 30 years old formalin fixed paraffin embedded (FFPE) tissue for PCR-based methylation analysis using SMART-MSP and MS-HRM

BACKGROUND: The High Resolution Melting (HRM) technology has recently been introduced as a rapid and robust analysis tool for the detection of DNA methylation. The methylation status of multiple tumor suppressor genes may serve as biomarkers for early cancer diagnostics, for prediction of prognosis...

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Detalles Bibliográficos
Autores principales: Kristensen, Lasse S, Wojdacz, Tomasz K, Thestrup, Britta B, Wiuf, Carsten, Hager, Henrik, Hansen, Lise Lotte
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2804714/
https://www.ncbi.nlm.nih.gov/pubmed/20025721
http://dx.doi.org/10.1186/1471-2407-9-453