Cargando…

Quality assessment of DNA derived from up to 30 years old formalin fixed paraffin embedded (FFPE) tissue for PCR-based methylation analysis using SMART-MSP and MS-HRM

BACKGROUND: The High Resolution Melting (HRM) technology has recently been introduced as a rapid and robust analysis tool for the detection of DNA methylation. The methylation status of multiple tumor suppressor genes may serve as biomarkers for early cancer diagnostics, for prediction of prognosis...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kristensen, Lasse S, Wojdacz, Tomasz K, Thestrup, Britta B, Wiuf, Carsten, Hager, Henrik, Hansen, Lise Lotte
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2804714/ https://www.ncbi.nlm.nih.gov/pubmed/20025721 http://dx.doi.org/10.1186/1471-2407-9-453

Ejemplares similares

Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue
por: Robertson, David, et al.
Publicado: (2008)

Toward deciphering proteomes of formalin-fixed paraffin-embedded (FFPE) tissues
por: Magdeldin, Sameh, et al.
Publicado: (2012)

Transcriptome Profiling of Archived Sectioned Formalin-Fixed Paraffin-Embedded (AS-FFPE) Tissue for Disease Classification
por: Kojima, Kensuke, et al.
Publicado: (2014)

miRNA expression profiling of formalin-fixed paraffin-embedded (FFPE) hereditary breast tumors
por: Tanić, Miljana, et al.
Publicado: (2014)

A Comparison of MGMT Testing by MSP and qMSP in Paired Snap-Frozen and Formalin-Fixed Paraffin-Embedded Gliomas
por: Lazarević, Milica, et al.
Publicado: (2023)

KRAS Mutation Detection in Paired Frozen and Formalin-Fixed Paraffin-Embedded (FFPE) Colorectal Cancer Tissues
por: Solassol, Jérome, et al.
Publicado: (2011)

Exome Enrichment and SOLiD Sequencing of Formalin Fixed Paraffin Embedded (FFPE) Prostate Cancer Tissue
por: Menon, Roopika, et al.
Publicado: (2012)

Aberrant MicroRNA Expression in Endometrial Carcinoma Using Formalin-Fixed Paraffin-Embedded (FFPE) Tissues
por: Lee, Taek Sang, et al.
Publicado: (2013)

An optimized workflow for microCT imaging of formalin‐fixed and paraffin‐embedded (FFPE) early equine embryos
por: Handschuh, Stephan, et al.
Publicado: (2022)

A novel method for analyzing formalin-fixed paraffin embedded (FFPE) tissue sections by mass spectrometry imaging
por: Aoki, Yutaka, et al.
Publicado: (2007)

Single-strand DNA library preparation improves sequencing of formalin-fixed and paraffin-embedded (FFPE) cancer DNA
por: Stiller, Mathias, et al.
Publicado: (2016)

Tissue lithography: Microscale dewaxing to enable retrospective studies on formalin-fixed paraffin-embedded (FFPE) tissue sections
por: Cors, Julien F., et al.
Publicado: (2017)

Whole exome sequencing (WES) on formalin-fixed, paraffin-embedded (FFPE) tumor tissue in gastrointestinal stromal tumors (GIST)
por: Astolfi, Annalisa, et al.
Publicado: (2015)

Formalin-Fixed Paraffin-Embedded (FFPE) samples are not a beneficial replacement for frozen tissues in fetal membrane microbiota research
por: Hockney, Rochelle, et al.
Publicado: (2022)

A New Intraepithelial γδ T-Lymphocyte Marker for Celiac Disease Classification in Formalin-Fixed Paraffin-Embedded (FFPE) Duodenal Biopsies
por: Popp, Alina, et al.
Publicado: (2020)

Technical challenges regarding the use of formalin-fixed paraffin embedded (FFPE) tissue specimens for the detection of bacterial alterations in colorectal cancer
por: Lam, Suk Yee, et al.
Publicado: (2021)

987. Identification of Fungal Pathogens In Formalin-Fixed Paraffin-Embedded (FFPE) Tissues by Targeted Next-Generation Sequencing (NGS) Technology
por: Totten, Marissa C, et al.
Publicado: (2023)

Gene-expression analysis of a colorectal cancer-specific discriminatory transcript set on formalin-fixed, paraffin-embedded (FFPE) tissue samples
por: Kalmár, Alexandra, et al.
Publicado: (2015)

Fabrication of Formalin-Fixed, Paraffin-Embedded (FFPE) Circulating Tumor Cell (CTC) Block Using a Hydrogel Core-Mediated Method
por: Lee, Tae Hee, et al.
Publicado: (2021)

Correction to: A New Intraepithelial γδ T-Lymphocyte Marker for Celiac Disease Classification in Formalin-Fixed Paraffin-Embedded (FFPE) Duodenal Biopsies
por: Popp, Alina, et al.
Publicado: (2020)

SARS-CoV-2 RNA detection in Formalin-Fixed Paraffin-Embedded (FFPE) tissue by droplet digital PCR (ddPCR)
por: Majumdar, Ramanath, et al.
Publicado: (2022)

Massively parallel sequencing of formalin-fixed paraffin-embedded tissues
por: Erlich, Rachel L, et al.
Publicado: (2012)

Virus characterization and discovery in formalin-fixed paraffin-embedded tissues
por: Bodewes, Rogier, et al.
Publicado: (2015)

Profiling chromatin accessibility in formalin-fixed paraffin-embedded samples
por: Zhang, Hua, et al.
Publicado: (2022)

Pre-Analytical Considerations for Successful Next-Generation Sequencing (NGS): Challenges and Opportunities for Formalin-Fixed and Paraffin-Embedded Tumor Tissue (FFPE) Samples
por: Arreaza, Gladys, et al.
Publicado: (2016)

A novel xylene-free deparaffinization method for the extraction of proteins from human derived formalin-fixed paraffin embedded (FFPE) archival tissue blocks
por: Mansour, Anthony, et al.
Publicado: (2014)

Identification and validation of differentially expressed transcripts by RNA-sequencing of formalin-fixed, paraffin-embedded (FFPE) lung tissue from patients with Idiopathic Pulmonary Fibrosis
por: Vukmirovic, Milica, et al.
Publicado: (2017)

Assessment of the quality of DNA from various formalin-fixed paraffin-embedded (FFPE) tissues and the use of this DNA for next-generation sequencing (NGS) with no artifactual mutation
por: Einaga, Naoki, et al.
Publicado: (2017)

A Non-Hazardous Deparaffinization Protocol Enables Quantitative Proteomics of Core Needle Biopsy-Sized Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens
por: Mitsa, Georgia, et al.
Publicado: (2022)

NGS Analysis of Liquid Biopsy (LB) and Formalin-Fixed Paraffin-Embedded (FFPE) Melanoma Samples Using Oncomine™ Pan-Cancer Cell-Free Assay
por: Olbryt, Magdalena, et al.
Publicado: (2021)

Dual NGS Comparative Analysis of Liquid Biopsy (LB) and Formalin-Fixed Paraffin-Embedded (FFPE) Samples of Non-Small Cell Lung Carcinoma (NSCLC)
por: Buburuzan, Laura, et al.
Publicado: (2022)

Expression profiling with RNA from formalin-fixed, paraffin-embedded material
por: Oberli, Andrea, et al.
Publicado: (2008)

Gene expression profiles of gliomas in formalin-fixed paraffin-embedded material
por: Gravendeel, L A M, et al.
Publicado: (2012)

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
por: Wiśniewski, Jacek R.
Publicado: (2013)

Molecular Markers for Prostate Cancer in Formalin-Fixed Paraffin-Embedded Tissues
por: Sequeiros, Tamara, et al.
Publicado: (2013)

Deep Clonal Profiling of Formalin Fixed Paraffin Embedded Clinical Samples
por: Holley, Tara, et al.
Publicado: (2012)

Epigenomic analysis of formalin-fixed paraffin-embedded samples by CUT&Tag
por: Henikoff, Steven, et al.
Publicado: (2023)

Improved RNA quality and TaqMan(® )Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials
por: Li, Jinghuan, et al.
Publicado: (2008)

Evaluation of formalin-fixed paraffin-embedded tissues in the proteomic analysis of parathyroid glands
por: Donadio, Elena, et al.
Publicado: (2011)

PrP(Sc )detection in formalin-fixed paraffin-embedded tissue by ELISA
por: Nicholson, Eric M, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_nruko180j4bv9n240nufq72vn4