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Overall Cdk activity modulates the DNA damage response in mammalian cells

In response to DNA damage, cells activate a phosphorylation-based signaling cascade known as the DNA damage response (DDR). One of the main outcomes of DDR activation is inhibition of cyclin-dependent kinase (Cdk) activity to restrain cell cycle progression until lesions are healed. Recent studies h...

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Detalles Bibliográficos
Autores principales: Cerqueira, Antonio, Santamaría, David, Martínez-Pastor, Bárbara, Cuadrado, Miriam, Fernández-Capetillo, Oscar, Barbacid, Mariano
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806328/
https://www.ncbi.nlm.nih.gov/pubmed/19995934
http://dx.doi.org/10.1083/jcb.200903033
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author Cerqueira, Antonio
Santamaría, David
Martínez-Pastor, Bárbara
Cuadrado, Miriam
Fernández-Capetillo, Oscar
Barbacid, Mariano
author_facet Cerqueira, Antonio
Santamaría, David
Martínez-Pastor, Bárbara
Cuadrado, Miriam
Fernández-Capetillo, Oscar
Barbacid, Mariano
author_sort Cerqueira, Antonio
collection PubMed
description In response to DNA damage, cells activate a phosphorylation-based signaling cascade known as the DNA damage response (DDR). One of the main outcomes of DDR activation is inhibition of cyclin-dependent kinase (Cdk) activity to restrain cell cycle progression until lesions are healed. Recent studies have revealed a reverse connection by which Cdk activity modulates processing of DNA break ends and DDR activation. However, the specific contribution of individual Cdks to this process remains poorly understood. To address this issue, we have examined the DDR in murine cells carrying a defined set of Cdks. Our results reveal that genome maintenance programs of postreplicative cells, including DDR, are regulated by the overall level of Cdk activity and not by specific Cdks.
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spelling pubmed-28063282010-06-14 Overall Cdk activity modulates the DNA damage response in mammalian cells Cerqueira, Antonio Santamaría, David Martínez-Pastor, Bárbara Cuadrado, Miriam Fernández-Capetillo, Oscar Barbacid, Mariano J Cell Biol Research Articles In response to DNA damage, cells activate a phosphorylation-based signaling cascade known as the DNA damage response (DDR). One of the main outcomes of DDR activation is inhibition of cyclin-dependent kinase (Cdk) activity to restrain cell cycle progression until lesions are healed. Recent studies have revealed a reverse connection by which Cdk activity modulates processing of DNA break ends and DDR activation. However, the specific contribution of individual Cdks to this process remains poorly understood. To address this issue, we have examined the DDR in murine cells carrying a defined set of Cdks. Our results reveal that genome maintenance programs of postreplicative cells, including DDR, are regulated by the overall level of Cdk activity and not by specific Cdks. The Rockefeller University Press 2009-12-14 /pmc/articles/PMC2806328/ /pubmed/19995934 http://dx.doi.org/10.1083/jcb.200903033 Text en © 2009 Cerqueira et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Cerqueira, Antonio
Santamaría, David
Martínez-Pastor, Bárbara
Cuadrado, Miriam
Fernández-Capetillo, Oscar
Barbacid, Mariano
Overall Cdk activity modulates the DNA damage response in mammalian cells
title Overall Cdk activity modulates the DNA damage response in mammalian cells
title_full Overall Cdk activity modulates the DNA damage response in mammalian cells
title_fullStr Overall Cdk activity modulates the DNA damage response in mammalian cells
title_full_unstemmed Overall Cdk activity modulates the DNA damage response in mammalian cells
title_short Overall Cdk activity modulates the DNA damage response in mammalian cells
title_sort overall cdk activity modulates the dna damage response in mammalian cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806328/
https://www.ncbi.nlm.nih.gov/pubmed/19995934
http://dx.doi.org/10.1083/jcb.200903033
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