Amplified RNA degradation in T7-amplification methods results in biased microarray hybridizations

BACKGROUND: The amplification of RNA with the T7-System is a widely used technique for obtaining increased amounts of RNA starting from limited material. The amplified RNA (aRNA) can subsequently be used for microarray hybridizations, warranting sufficient signal for image analysis. We describe here...

Descripción completa

Detalles Bibliográficos
Autores principales: Spiess, Andrej-Nikolai, Mueller, Nadine, Ivell, Richard
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC280674/
https://www.ncbi.nlm.nih.gov/pubmed/14606961
http://dx.doi.org/10.1186/1471-2164-4-44

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC280674/
https://www.ncbi.nlm.nih.gov/pubmed/14606961
http://dx.doi.org/10.1186/1471-2164-4-44

Ejemplares similares