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Amplified RNA degradation in T7-amplification methods results in biased microarray hybridizations
BACKGROUND: The amplification of RNA with the T7-System is a widely used technique for obtaining increased amounts of RNA starting from limited material. The amplified RNA (aRNA) can subsequently be used for microarray hybridizations, warranting sufficient signal for image analysis. We describe here...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2003
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC280674/ https://www.ncbi.nlm.nih.gov/pubmed/14606961 http://dx.doi.org/10.1186/1471-2164-4-44 |