Cargando…

Selection of reference genes for quantitative RT-PCR studies in Rhipicephalus (Boophilus) microplus and Rhipicephalus appendiculatus ticks and determination of the expression profile of Bm86

BACKGROUND: For accurate and reliable gene expression analysis, normalization of gene expression data against reference genes is essential. In most studies on ticks where (semi-)quantitative RT-PCR is employed, normalization occurs with a single reference gene, usually β-actin, without validation of...

Descripción completa

Detalles Bibliográficos
Autores principales: Nijhof, Ard M, Balk, Jesper A, Postigo, Milagros, Jongejan, Frans
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2809063/
https://www.ncbi.nlm.nih.gov/pubmed/20040102
http://dx.doi.org/10.1186/1471-2199-10-112