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Selection of reference genes for quantitative RT-PCR studies in Rhipicephalus (Boophilus) microplus and Rhipicephalus appendiculatus ticks and determination of the expression profile of Bm86

BACKGROUND: For accurate and reliable gene expression analysis, normalization of gene expression data against reference genes is essential. In most studies on ticks where (semi-)quantitative RT-PCR is employed, normalization occurs with a single reference gene, usually β-actin, without validation of...

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Detalles Bibliográficos
Autores principales:	Nijhof, Ard M, Balk, Jesper A, Postigo, Milagros, Jongejan, Frans
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2809063/ https://www.ncbi.nlm.nih.gov/pubmed/20040102 http://dx.doi.org/10.1186/1471-2199-10-112

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