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Modification of N-glycosylation sites allows secretion of bacterial chondroitinase ABC from mammalian cells
Although many eukaryotic proteins have been secreted by transfected bacterial cells, little is known about how a bacterial protein is treated as it passes through the secretory pathway when expressed in a eukaryotic cell. The eukaryotic N-glycosylation system could interfere with folding and secreti...
Autores principales: | Muir, Elizabeth M., Fyfe, Ian, Gardiner, Sonya, Li, Li, Warren, Philippa, Fawcett, James W., Keynes, Roger J., Rogers, John H. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science Publishers
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2809921/ https://www.ncbi.nlm.nih.gov/pubmed/19900493 http://dx.doi.org/10.1016/j.jbiotec.2009.11.002 |
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