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Zymographic assay of plant diamine oxidase on entrapped peroxidase polyacrylamide gel electrophoresis. A study of stability to proteolysis

A zymographic assay of diamine oxidase (DAO, histaminase, EC 1.4.3.6), based on a coupled peroxidase reaction, and its behavior at proteolysis in simulated gastric and intestinal conditions, are described. The DAO activity from a vegetal extract of Lathyrus sativus seedlings was directly determined...

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Detalles Bibliográficos
Autores principales: Calinescu, Carmen, Federico, Rodolfo, Mondovi, Bruno, Mateescu, Mircea Alexandru
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2809943/
https://www.ncbi.nlm.nih.gov/pubmed/20091155
http://dx.doi.org/10.1007/s00216-009-3306-7