Selective control of primer usage in multiplex one-step reverse transcription PCR

BACKGROUND: Multiplex RT-PCR is a valuable technique used for pathogen identification, disease detection and relative quantification of gene expression. The simplification of this protocol into a one-step procedure saves time and reagents. However, intensive PCR optimization is often required to ove...

Descripción completa

Detalles Bibliográficos
Autores principales: Hidalgo Ashrafi, Elena, Yee, Joyclyn, Paul, Natasha
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Research article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811113/
https://www.ncbi.nlm.nih.gov/pubmed/20042116
http://dx.doi.org/10.1186/1471-2199-10-113

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811113/
https://www.ncbi.nlm.nih.gov/pubmed/20042116
http://dx.doi.org/10.1186/1471-2199-10-113

Ejemplares similares