In vitro reconstruction of tetronate RK-682 biosynthesis

The protein phosphatase inhibitor RK-682 is one of a number of potentially valuable tetronate polyketide natural products. Understanding how the tetronate ring is formed has been frustrated by the inaccessibility of the putative substrates. We report the heterologous expression of rk genes in Saccha...

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Detalles Bibliográficos
Autores principales: Sun, Yuhui, Hahn, Frank, Demydchuk, Yuliya, Chettle, James, Tosin, Manuela, Osada, Hiroyuki, Leadlay, Peter F.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811812/
https://www.ncbi.nlm.nih.gov/pubmed/20081823
http://dx.doi.org/10.1038/nchembio.285
Descripción
Sumario:The protein phosphatase inhibitor RK-682 is one of a number of potentially valuable tetronate polyketide natural products. Understanding how the tetronate ring is formed has been frustrated by the inaccessibility of the putative substrates. We report the heterologous expression of rk genes in Saccharopolyspora erythraea and reconstitution of the RK-682 pathway using recombinant enzymes, and show that RkD is the enzyme required for RK-682 formation from acyl carrier protein-bound substrates.

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