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Serum is not required for ex vivo IFN-γ ELISPOT: a collaborative study of different protocols from the European CIMT Immunoguiding Program

The Cancer Immunotherapy Immunoguiding Program has conducted an IFN-γ ELISPOT proficiency panel to examine the influence of serum supplementation of test media on assay performance. Sixteen European laboratories analyzed the same PBMC samples using different locally established protocols. Participan...

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Detalles Bibliográficos
Autores principales: Mander, A., Gouttefangeas, C., Ottensmeier, C., Welters, M. J. P., Low, L., van der Burg, S. H., Britten, C. M.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2813523/
https://www.ncbi.nlm.nih.gov/pubmed/20052465
http://dx.doi.org/10.1007/s00262-009-0814-4