Visualisation of PCNA Monoubiquitination In Vivo by Single Pass Spectral Imaging FRET Microscopy

Monoubiquitination of the DNA sliding clamp, PCNA, plays a central role in the control of damage bypass during replication. By combining a widely-spaced FRET donor/acceptor pair (CFP and mRFP) with spectral imaging, we have developed a simple method for the visualisation of PCNA monoubiquitination i...

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Detalles Bibliográficos
Autores principales: Batters, Christopher, Zhu, Hannah, Sale, Julian E.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2814862/
https://www.ncbi.nlm.nih.gov/pubmed/20126408
http://dx.doi.org/10.1371/journal.pone.0009008

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2814862/
https://www.ncbi.nlm.nih.gov/pubmed/20126408
http://dx.doi.org/10.1371/journal.pone.0009008

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