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From Dynamic Live Cell Imaging to 3D Ultrastructure: Novel Integrated Methods for High Pressure Freezing and Correlative Light-Electron Microscopy

BACKGROUND: In cell biology, the study of proteins and organelles requires the combination of different imaging approaches, from live recordings with light microscopy (LM) to electron microscopy (EM). METHODOLOGY: To correlate dynamic events in adherent cells with both ultrastructural and 3D informa...

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Detalles Bibliográficos
Autores principales: Spiegelhalter, Coralie, Tosch, Valérie, Hentsch, Didier, Koch, Marc, Kessler, Pascal, Schwab, Yannick, Laporte, Jocelyn
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815783/
https://www.ncbi.nlm.nih.gov/pubmed/20140253
http://dx.doi.org/10.1371/journal.pone.0009014