Quantitative analysis of ribosome–mRNA complexes at different translation stages

Inhibition of primer extension by ribosome–mRNA complexes (toeprinting) is a proven and powerful technique for studying mechanisms of mRNA translation. Here we have assayed an advanced toeprinting approach that employs fluorescently labeled DNA primers, followed by capillary electrophoresis utilizin...

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Detalles Bibliográficos
Autores principales: Shirokikh, Nikolay E., Alkalaeva, Elena Z., Vassilenko, Konstantin S., Afonina, Zhanna A., Alekhina, Olga M., Kisselev, Lev L., Spirin, Alexander S.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817456/
https://www.ncbi.nlm.nih.gov/pubmed/19910372
http://dx.doi.org/10.1093/nar/gkp1025

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817456/
https://www.ncbi.nlm.nih.gov/pubmed/19910372
http://dx.doi.org/10.1093/nar/gkp1025

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