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Quantitative analysis of ribosome–mRNA complexes at different translation stages

Inhibition of primer extension by ribosome–mRNA complexes (toeprinting) is a proven and powerful technique for studying mechanisms of mRNA translation. Here we have assayed an advanced toeprinting approach that employs fluorescently labeled DNA primers, followed by capillary electrophoresis utilizin...

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Detalles Bibliográficos
Autores principales:	Shirokikh, Nikolay E., Alkalaeva, Elena Z., Vassilenko, Konstantin S., Afonina, Zhanna A., Alekhina, Olga M., Kisselev, Lev L., Spirin, Alexander S.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2010
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817456/ https://www.ncbi.nlm.nih.gov/pubmed/19910372 http://dx.doi.org/10.1093/nar/gkp1025

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