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Exploring the Roles of the Metal Ions in Escherichia coli Copper Amine Oxidase(,)
[Image: see text] To investigate the role of the active site copper in Escherichia coli copper amine oxidase (ECAO), we initiated a metal-substitution study. Copper reconstitution of ECAO (Cu-ECAO) restored only ∼12% wild-type activity as measured by k(cat(amine)). Treatment with EDTA, to remove exo...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817917/ https://www.ncbi.nlm.nih.gov/pubmed/20052994 http://dx.doi.org/10.1021/bi901738k |