Systematic overrepresentation of DNA termini and underrepresentation of subterminal regions among sequencing templates prepared from hydrodynamically sheared linear DNA molecules

BACKGROUND: Analysis of fungal genome sequence assemblies reveals that telomeres are poorly represented even though telomeric reads tend to be superabundant. We surmised that the problem might lie in the DNA shearing conditions used to create clone libraries for genome sequencing. RESULTS: A shotgun...

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Detalles Bibliográficos
Autores principales: Schwartz, Sherri L, Farman, Mark L
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2824731/
https://www.ncbi.nlm.nih.gov/pubmed/20122283
http://dx.doi.org/10.1186/1471-2164-11-87

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2824731/
https://www.ncbi.nlm.nih.gov/pubmed/20122283
http://dx.doi.org/10.1186/1471-2164-11-87

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