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A Flow Cytometry-Based FRET Assay to Identify and Analyse Protein-Protein Interactions in Living Cells
BACKGROUND: Försters resonance energy transfer (FRET) microscopy is widely used for the analysis of protein interactions in intact cells. However, FRET microscopy is technically challenging and does not allow assessing interactions in large cell numbers. To overcome these limitations we developed a...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2825263/ https://www.ncbi.nlm.nih.gov/pubmed/20179761 http://dx.doi.org/10.1371/journal.pone.0009344 |