Cargando…

A Flow Cytometry-Based FRET Assay to Identify and Analyse Protein-Protein Interactions in Living Cells

BACKGROUND: Försters resonance energy transfer (FRET) microscopy is widely used for the analysis of protein interactions in intact cells. However, FRET microscopy is technically challenging and does not allow assessing interactions in large cell numbers. To overcome these limitations we developed a...

Descripción completa

Detalles Bibliográficos
Autores principales:	Banning, Carina, Votteler, Jörg, Hoffmann, Dirk, Koppensteiner, Herwig, Warmer, Martin, Reimer, Rudolph, Kirchhoff, Frank, Schubert, Ulrich, Hauber, Joachim, Schindler, Michael
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2010
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2825263/ https://www.ncbi.nlm.nih.gov/pubmed/20179761 http://dx.doi.org/10.1371/journal.pone.0009344

Ejemplares similares

Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay
por: Winkler, Michael, et al.
Publicado: (2019)

Formation of Trans-Activation Competent HIV-1 Rev:RRE Complexes Requires the Recruitment of Multiple Protein Activation Domains
por: Hoffmann, Dirk, et al.
Publicado: (2012)

Vpu serine 52 dependent counteraction of tetherin is required for HIV-1 replication in macrophages, but not in ex vivo human lymphoid tissue
por: Schindler, Michael, et al.
Publicado: (2010)

Flow cytometry based-FRET: basics, novel developments and future perspectives
por: Lim, JiaWen, et al.
Publicado: (2022)

Flow cytometry-based FRET identifies binding intensities in PPARγ1 protein-protein interactions in living cells
por: Trümper, Verena, et al.
Publicado: (2019)

Solution structure of the Equine Infectious Anemia Virus p9 protein: a rationalization of its different ALIX binding requirements compared to the analogous HIV-p6 protein
por: Sharma, Alok, et al.
Publicado: (2009)

Solution structure of the equine infectious anemia virus p9 protein: a rationalization of its different ALIX binding requirements compared to the analogous HIV-p6 protein
por: Sharma, Alok, et al.
Publicado: (2009)

The Ebola Virus Glycoprotein and HIV-1 Vpu Employ Different Strategies to Counteract the Antiviral Factor Tetherin
por: Kühl, Annika, et al.
Publicado: (2011)

Dual FRET assay for detecting receptor protein interaction with DNA
por: Krusiński, Tomasz, et al.
Publicado: (2010)

HIV-1 Replication in Human Immune Cells Is Independent of TAR DNA Binding Protein 43 (TDP-43) Expression
por: Nehls, Julia, et al.
Publicado: (2014)

Development of FRET Assay into Quantitative and High-throughput Screening Technology Platforms for Protein–Protein Interactions
por: Song, Yang, et al.
Publicado: (2010)

A FRET-Based Assay for the Identification of PCNA Inhibitors
por: Hardebeck, Sarah, et al.
Publicado: (2023)

Exonuclease III protection assay with FRET probe for detecting DNA-binding proteins
por: Wang, Jinke, et al.
Publicado: (2005)

Quantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution
por: Liao, Jiayu, et al.
Publicado: (2021)

Stimulant drug effects on touchscreen automated paired-associates learning (PAL) in rats
por: Roschlau, Corinna, et al.
Publicado: (2016)

Photoswitching FRET to monitor protein–protein interactions
por: Rainey, Kristin H., et al.
Publicado: (2019)

DSE-FRET: A new anticancer drug screening assay for DNA binding proteins
por: Miyagi, Toru, et al.
Publicado: (2014)

FRET-based assay to screen inhibitors of HIV-1 reverse transcriptase and nucleocapsid protein
por: Sharma, Kamal K., et al.
Publicado: (2016)

Practical and reliable FRET/FLIM pair of fluorescent proteins
por: Shcherbo, Dmitry, et al.
Publicado: (2009)

Abolishing HIV-1 infectivity using a polypurine tract-specific G-quadruplex-forming oligonucleotide
por: Voges, Maike, et al.
Publicado: (2016)

Macrophages and their relevance in Human Immunodeficiency Virus Type I infection
por: Koppensteiner, Herwig, et al.
Publicado: (2012)

Action-FRET of a Gaseous Protein
por: Daly, Steven, et al.
Publicado: (2016)

Protein–Protein Affinity Determination by Quantitative FRET Quenching
por: Jiang, Ling, et al.
Publicado: (2019)

Liposomal FRET Assay Identifies Potent Drug‐Like Inhibitors of the Ceramide Transport Protein (CERT)
por: Samaha, Doaa, et al.
Publicado: (2020)

Role of the Medial Orbitofrontal Cortex and Ventral Tegmental Area in Effort-Related Responding
por: Münster, Alexandra, et al.
Publicado: (2020)

Potent in vitro antiviral activity of Cistus incanus extract against HIV and Filoviruses targets viral envelope proteins
por: Rebensburg, Stephanie, et al.
Publicado: (2016)

SARS-CoV-2 Nucleocapsid Protein TR-FRET Assay Amenable to High Throughput Screening
por: Gorshkov, Kirill, et al.
Publicado: (2022)

Development of a steady-state FRET-based assay to identify inhibitors of the Keap1-Nrf2 protein–protein interaction
por: Schaap, Marjolein, et al.
Publicado: (2013)

A FRET flow cytometry method for monitoring cytosolic and glycosomal glucose in living kinetoplastid parasites
por: Voyton, Charles M., et al.
Publicado: (2018)

Features of Protein Unfolding Transitions and Their Relation to Domain Topology Probed by Single-Molecule FRET
por: Bustorff, Nuno, et al.
Publicado: (2023)

Quantification of protein–lipid selectivity using FRET
por: Loura, Luís M. S., et al.
Publicado: (2009)

A Guide to Fluorescent Protein FRET Pairs
por: Bajar, Bryce T., et al.
Publicado: (2016)

Single‐Molecule FRET of Membrane Transport Proteins
por: Bartels, Kim, et al.
Publicado: (2021)

An enhanced triple fluorescence flow-cytometry-based assay shows differential activation of the Notch signaling pathway by human papillomavirus E6 proteins
por: Lim, JiaWen, et al.
Publicado: (2022)

Mutation of the Highly Conserved Ser-40 of the HIV-1 p6 Gag Protein to Phe Causes the Formation of a Hydrophobic Patch, Enhances Membrane Association, and Polyubiquitination of Gag
por: Hahn, Friedrich, et al.
Publicado: (2014)

Interbase-FRET binding assay for pre-microRNAs
por: Bood, Mattias, et al.
Publicado: (2021)

Improving FRET dynamic range with bright green and red fluorescent proteins
por: Lam, Amy, et al.
Publicado: (2012)

TR-FRET Assays of Huntingtin Protein Fragments Reveal Temperature and PolyQ Length-Dependent Conformational Changes
por: Cui, Xiaotian, et al.
Publicado: (2014)

A SARS-CoV-2 nucleocapsid protein TR-FRET assay amenable to high-throughput screening
por: Gorshkov, Kirill, et al.
Publicado: (2021)

Comparison of Aggregometry with Flow Cytometry for the Assessment of Agonists´-Induced Platelet Reactivity in Patients on Dual Antiplatelet Therapy
por: Gremmel, Thomas, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_2kt2bp2ibv253e56pfahv3s1nr