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Flavonoid profiling and transcriptome analysis reveals new gene–metabolite correlations in tubers of Solanum tuberosum L.

Anthocyanin content of potato tubers is a trait that is attracting increasing attention as the potential nutritional benefits of this class of compound become apparent. However, our understanding of potato tuber anthocyanin accumulation is not complete. The aim of this study was to use a potato micr...

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Autores principales: Stushnoff, Cecil, Ducreux, Laurence J. M., Hancock, Robert D., Hedley, Pete E., Holm, David G., McDougall, Gordon J., McNicol, James W., Morris, Jenny, Morris, Wayne L., Sungurtas, Julie A., Verrall, Susan R., Zuber, Tatiana, Taylor, Mark A.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826661/
https://www.ncbi.nlm.nih.gov/pubmed/20110266
http://dx.doi.org/10.1093/jxb/erp394
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author Stushnoff, Cecil
Ducreux, Laurence J. M.
Hancock, Robert D.
Hedley, Pete E.
Holm, David G.
McDougall, Gordon J.
McNicol, James W.
Morris, Jenny
Morris, Wayne L.
Sungurtas, Julie A.
Verrall, Susan R.
Zuber, Tatiana
Taylor, Mark A.
author_facet Stushnoff, Cecil
Ducreux, Laurence J. M.
Hancock, Robert D.
Hedley, Pete E.
Holm, David G.
McDougall, Gordon J.
McNicol, James W.
Morris, Jenny
Morris, Wayne L.
Sungurtas, Julie A.
Verrall, Susan R.
Zuber, Tatiana
Taylor, Mark A.
author_sort Stushnoff, Cecil
collection PubMed
description Anthocyanin content of potato tubers is a trait that is attracting increasing attention as the potential nutritional benefits of this class of compound become apparent. However, our understanding of potato tuber anthocyanin accumulation is not complete. The aim of this study was to use a potato microarray to investigate gene expression patterns associated with the accumulation of purple tuber anthocyanins. The advanced potato selections, CO97216-3P/PW and CO97227-2P/PW, developed by conventional breeding procedures, produced tubers with incomplete expression of tuber flesh pigmentation. This feature permits sampling pigmented and non-pigmented tissues from the same tubers, in essence, isolating the factors responsible for pigmentation from confounding genetic, environmental, and developmental effects. An examination of the transcriptome, coupled with metabolite data from purple pigmented sectors and from non-pigmented sectors of the same tuber, was undertaken to identify these genes whose expression correlated with elevated or altered polyphenol composition. Combined with a similar study using eight other conventional cultivars and advanced selections with different pigmentation, it was possible to produce a refined list of only 27 genes that were consistently differentially expressed in purple tuber tissues compared with white. Within this list are several new candidate genes that are likely to impact on tuber anthocyanin accumulation, including a gene encoding a novel single domain MYB transcription factor.
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spelling pubmed-28266612010-02-24 Flavonoid profiling and transcriptome analysis reveals new gene–metabolite correlations in tubers of Solanum tuberosum L. Stushnoff, Cecil Ducreux, Laurence J. M. Hancock, Robert D. Hedley, Pete E. Holm, David G. McDougall, Gordon J. McNicol, James W. Morris, Jenny Morris, Wayne L. Sungurtas, Julie A. Verrall, Susan R. Zuber, Tatiana Taylor, Mark A. J Exp Bot Research Papers Anthocyanin content of potato tubers is a trait that is attracting increasing attention as the potential nutritional benefits of this class of compound become apparent. However, our understanding of potato tuber anthocyanin accumulation is not complete. The aim of this study was to use a potato microarray to investigate gene expression patterns associated with the accumulation of purple tuber anthocyanins. The advanced potato selections, CO97216-3P/PW and CO97227-2P/PW, developed by conventional breeding procedures, produced tubers with incomplete expression of tuber flesh pigmentation. This feature permits sampling pigmented and non-pigmented tissues from the same tubers, in essence, isolating the factors responsible for pigmentation from confounding genetic, environmental, and developmental effects. An examination of the transcriptome, coupled with metabolite data from purple pigmented sectors and from non-pigmented sectors of the same tuber, was undertaken to identify these genes whose expression correlated with elevated or altered polyphenol composition. Combined with a similar study using eight other conventional cultivars and advanced selections with different pigmentation, it was possible to produce a refined list of only 27 genes that were consistently differentially expressed in purple tuber tissues compared with white. Within this list are several new candidate genes that are likely to impact on tuber anthocyanin accumulation, including a gene encoding a novel single domain MYB transcription factor. Oxford University Press 2010-02 2010-01-28 /pmc/articles/PMC2826661/ /pubmed/20110266 http://dx.doi.org/10.1093/jxb/erp394 Text en © 2010 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Stushnoff, Cecil
Ducreux, Laurence J. M.
Hancock, Robert D.
Hedley, Pete E.
Holm, David G.
McDougall, Gordon J.
McNicol, James W.
Morris, Jenny
Morris, Wayne L.
Sungurtas, Julie A.
Verrall, Susan R.
Zuber, Tatiana
Taylor, Mark A.
Flavonoid profiling and transcriptome analysis reveals new gene–metabolite correlations in tubers of Solanum tuberosum L.
title Flavonoid profiling and transcriptome analysis reveals new gene–metabolite correlations in tubers of Solanum tuberosum L.
title_full Flavonoid profiling and transcriptome analysis reveals new gene–metabolite correlations in tubers of Solanum tuberosum L.
title_fullStr Flavonoid profiling and transcriptome analysis reveals new gene–metabolite correlations in tubers of Solanum tuberosum L.
title_full_unstemmed Flavonoid profiling and transcriptome analysis reveals new gene–metabolite correlations in tubers of Solanum tuberosum L.
title_short Flavonoid profiling and transcriptome analysis reveals new gene–metabolite correlations in tubers of Solanum tuberosum L.
title_sort flavonoid profiling and transcriptome analysis reveals new gene–metabolite correlations in tubers of solanum tuberosum l.
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826661/
https://www.ncbi.nlm.nih.gov/pubmed/20110266
http://dx.doi.org/10.1093/jxb/erp394
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